We have conducted a survey program on the awarnance of HPV vaccine of cervical cancer in common people. Methods: For this survey we perform 2 steps. First we made a questionnaires in which we ask to female of different belongs to different education field either they are married or not. Secondly we gone in the different hospitals of Karachi and observe treatment, diagnosis, vaccination availability and frequency of cervical cancer. Results:From questionnaire we observed that only 1 % female are aware about cervical cancer and its vaccine i.e. HPV, even female belongs medical field are not aware about it. Form hospital survey we observed that frequency of cervical cancer is very less but in Shaukat Khanum hospital 90 cases reported out of 1803 cancer. The given treatment is radiology, chemotherapy and surgery. Conclusion: From this survey we conclude that the awarnance of people on cervical cancer and its vaccination is negligible.

The purpose of this study is to analyze and assess the risk associated with the presence of aflatoxins in food and feed. Aflatoxins are produced by Aspergillus flavus and Aspergillus parasiticus and they are distributed worldwide. Conclusions: Aflatoxins are a potential danger for both animal and human health. Attention should be paid to the potentially dangerous synergistic or additive effects on animal health arising from the presence of more than one mycotoxin in feed. Given the ubiquity of mycotoxins in the world, is also necessary to develop an effective program of risk management, which will be crucial to reduce costs and economic losses in farms associated with the risk of pollution.

Myelin oligodendrocyte glycoprotein (MOG) is considered as a biokmaker in multiple sclerosis patients, MOG is unique to the brain, it is located on the outer lamellae of oligodendroglial membranes and myelin and it is highly immunogenic. Objective: In the present study an evaluation of serum anti-MOG antibody in relapsing remitting multiple sclerosis (RRMS) patients was carried out. Method: Serum levels of anti-MOG were measured by ELISA method after venous blood was collected from forty MS patients and forty healthy subjects as control group. Absorbance was read at a wave length of 450nm using ELISA reader. Anti-MOG antibody level was then calculated by plotting the optical density (O.D.) of each sample against the concentration in the standard curve. Results: A significant difference between serum Anti-MOG concentration in patients 554.85 ± 39.73pg/ml and the control group with 315.20 ± 28.49pg/ml, (p=0. 0211) was found. Also Anti-MOG has a negative correlation between age and concentration (r= -0.12) and significant positive correlation between expanded disability status scale (EDSS) and concentration (r= 0.28). Conclusion: Data for serum level of Anti-MOG Ab are present in this study. They are a basis for the role of Anti-MOG in multiple sclerosis patients. The present findings showed an increased levels of anti-MOG antibody in patient group which may suggest the demyelinating role of anti-MOG antibody and the importance of humoral immunity in relapsing remitting multiple sclerosis patients. Therefore the data found in this work might be used for further study on the importance of humoral immunity in autoimmune disease like MS.